کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6141805 1227314 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
N-terminally truncated C protein, CNΔ25, of human parainfluenza virus type 3 is a potent inhibitor of viral replication
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
N-terminally truncated C protein, CNΔ25, of human parainfluenza virus type 3 is a potent inhibitor of viral replication
چکیده انگلیسی
The C protein of human parainfluenza virus type 3 (HPIV3) is a multifunctional accessory protein that inhibits viral transcription and interferon (IFN) signaling. In the present study, we found that removal of N-terminal 25 or 50 amino acid residues from the C protein (CNΔ25 or CNΔ50) totally abolished viral RNA synthesis in the HPIV3 minigenome system. Further N-terminal or C-terminal deletion impaired the inhibitory ability of CNΔ25 and CNΔ50. Subsequent mutagenesis analysis suggested that the N-terminal-charged amino acid residues (K3, K6, K12, E16, and R24) contribute to the higher inhibition caused by CNΔ25 than the C protein. Consistent with viral RNA synthesis inhibition, the growth of HPIV3 was significantly decreased by 5 logs in HeLa-derived cell line expressing CNΔ25. Interestingly, replication of respiratory syncytial virus (RSV), another important respiratory tract pathogen, was also strongly inhibited in the presence of CNΔ25. These findings provide a promising potential to use CNΔ25 as an antiviral agent against the clinically important respiratory tract diseases caused by HPIV3 and RSV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 394, Issue 1, 10 November 2009, Pages 143-148
نویسندگان
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