کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6141833 1227320 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Deletions within the 5′UTR of coxsackievirus B3: Consequences for virus translation and replication
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Deletions within the 5′UTR of coxsackievirus B3: Consequences for virus translation and replication
چکیده انگلیسی
Key features of an ideal RNA-based vaccine against coxsackievirus B3 (CVB3) are (i) limited genome replication/virus production (to minimize vaccine-related pathology) and (ii) abundant virus protein synthesis (to maximize immunogenicity). These attributes may apply to CVB3 RNAs lacking up to 250 nucleotides (nt) from their 5′ terminus; these RNAs do not give rise to infectious progeny, but they have been reported to retain the entire CVB3 IRES (mapped to nt ∼ 432-639) and to produce large quantities of viral protein in transfected cells. Here, we constructed five 5′ RNA deletion variants that, to our surprise, failed to protect against CVB3 challenge. We investigated the reasons for this failure and conclude that (i) a 5′ terminal deletion as short as 32 nt abolishes CVB3 RNA replication in transfected cells; (ii) this deleted RNA, and others with longer deletions, do not direct abundant protein synthesis in transfected cells, probably as a consequence of their replicative incapacity; and (iii) the CVB3 IRES is substantially larger than previously thought, and its 5′ boundary lies between residues 76 and 125, very closely approximating that of the poliovirus IRES.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 360, Issue 1, 30 March 2007, Pages 120-128
نویسندگان
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