کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6141950 1227358 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Productive infection of human immunodeficiency virus type 1 in dendritic cells requires fusion-mediated viral entry
چکیده انگلیسی

Human immunodeficiency virus type 1 (HIV-1) enters dendritic cells (DCs) through endocytosis and viral receptor-mediated fusion. Although endocytosis-mediated HIV-1 entry can generate productive infection in certain cell types, including human monocyte-derived macrophages, productive HIV-1 infection in DCs appears to be dependent on fusion-mediated viral entry. It remains to be defined whether endocytosed HIV-1 in DCs can initiate productive infection. Using HIV-1 infection and cellular fractionation assays to measure productive viral infection and entry, here we show that HIV-1 enters monocyte-derived DCs predominately through endocytosis; however, endocytosed HIV-1 cannot initiate productive HIV-1 infection in DCs. In contrast, productive HIV-1 infection in DCs requires fusion-mediated viral entry. Together, these results provide functional evidence in understanding HIV-1 cis-infection of DCs, suggesting that different pathways of HIV-1 entry into DCs determine the outcome of viral infection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 375, Issue 2, 5 June 2008, Pages 442-451
نویسندگان
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