کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6142152 | 1594340 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The deletion of residues 268-292 of E1 impairs the ability of HCV envelope proteins to induce pore formation
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کلمات کلیدی
CCADPXHCVppNBD-PEconvex constraint analysisRh-PE - با Rh-PELipid-protein interaction - تعامل لیپید-پروتئینegg yolk phosphatidylglycerol - زرده تخم مرغ فسفاتیدیل گلیسرولMembrane fusion - فیوژن غشاییAnts - مورچه، مورHCV - هپاتیت سیHepatitis C virus - هپاتیت سیViral envelope proteins - پروتئین پاکت های ویروسی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The deletion of residues 268-292 of E1 impairs the ability of HCV envelope proteins to induce pore formation The deletion of residues 268-292 of E1 impairs the ability of HCV envelope proteins to induce pore formation](/preview/png/6142152.png)
چکیده انگلیسی
We have obtained a chimeric protein containing the ectodomains of hepatitis C virus (HCV) envelope proteins but lacking the region 268-292 of E1. All its structural properties are coincident with those of the corresponding full length chimera. The deleted and entire chimeras were compared in terms of their membrane destabilizing properties. No differences were found in their ability to induce vesicle aggregation and lipid mixing but the deleted chimera showed a reduced capacity to promote leakage. The role of the deletion was also studied by obtaining HCV pseudoparticles (HCVpp). Both E1 and E2, and also the E1 deleted mutant, were incorporated into HCVpp to a similar level. However, HCVpp containing the E1 deleted protein are almost unable to infect Huh7 cells. These results point to the involvement of the region 268-292 in the formation of pores in the membrane necessary for the complete fusion of the membranes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 217, 2 June 2016, Pages 63-70
Journal: Virus Research - Volume 217, 2 June 2016, Pages 63-70
نویسندگان
Laura Lombana, Sara Ortega-Atienza, Julián Gómez-Gutiérrez, Belén Yélamos, Darrell L. Peterson, Francisco Gavilanes,