A prime/boost strategy by DNA/fowlpox recombinants expressing a mutant E7 protein for the immunotherapy of HPV-associated cancers

► The development of a therapeutic vaccine against HPV remains a high priority. ► HPV16 E7GGG mutated protein expression by DNA/FP recombinants can be a safe strategy. ► FPE7GGG recombinant expresses the transgene in different cell lines correctly. ► The combined use of the recombinants showed a significant delay in tumor growth.