Characterization of non-polio enterovirus isolates from acute flaccid paralysis children in Pakistan reflects a new genotype of EV-107

► Identification of non-polio enteroviruses associated with acute flaccid paralysis circulating in Pakistan; a polio endemic country. ► Emergence of a new genotype of enterovirus-107 in Pakistan. ► Our findings substantiate the use of molecular tools to successfully identify the virus variant as well as novel genotypes.