Analysis of the pX region of bovine leukemia virus in different clinical stages of Enzootic Bovine Leukemia in Argentine Holstein cattle

► We compared the wild-type proviral pXBLV region at different stages of BLV infected Argentine Holstein. ► The proviral pX region of 12 cattle was amplified by PCR. ► This region was sequenced, aligned and compared both with each other and with the reference sequence. ► Applying hierarchical clustering two clusters were obtained, not allowing differentiate by clinical stage. ► We found no amino acid strict differences between the categories of BLV-positive Argentine Holsteins.