NC-mediated nucleolar localization of retroviral gag proteins

► The NC domains of the RSV and HIV Gag proteins contain nucleolar localization signals. ► RSV Gag can accumulate in nucleoli when CRM1-mediated nuclear export is inhibited. ► Nuclear RSV Gag proteins are mobile, cycling rapidly between nucleoli and nucleoplasm. ► A subpopulation of Rev-dependent HIV-1 Gag localizes to nucleoli.