Gp96 enhances the immunogenicity of subunit vaccine of porcine reproductive and respiratory syndrome virus

► We construct two epitope-based proteins (Cp1/Cp2) as subunit vaccine of PRRSV. ► We use the N terminal 22-370 aa of porcine gp96 (Gp96N) as the adjuvant. ► Gp96N can enhance humoral and cellular immunity elicited by Cp1/Cp2. ► Gp96N could improve innate and adaptive immunity with Th1 biased immune response.