Subunit vaccine formulations based on recombinant envelope proteins of Chikungunya virus elicit balanced Th1/Th2 response and virus-neutralizing antibodies in mice

► The immunogenic potential of recombinant CHIKV envelope proteins was evaluated in mice. ► Recombinant protein elicited a strong humoral response and a balanced Th1/Th2 response. ► In vivo and in vitro efficacy of various adjuvant based formulations was also evaluated. ► Recombinant CHIKV antigens can be proposed as potent subunit vaccine candidates.