Australian monocot-infecting mastrevirus diversity rivals that in Africa

► 41 virus mastrevirus isolates found in naturalised and native Australian grasses. ► Identified four new species and two of these are highly divergent and are most closely related to the African streak viruses. ► Patterns of inter- and intra-species recombination and the recombination hotspots mirror those of all other mastreviruses. ► Australian monocot-infecting mastrevirus diversity rivals that in Africa.