Phenotypic grouping of 141 BmNPVs lacking viral gene sequences

► We constructed a series of gene knockout BmNPVs (KOVs) for each of 141 genes using BmNPV T3 bacmid system. ► KOVs were subdivided into four phenotypic types by polyhedrin promoter-driven EGFP expression and infectious behavior. ► The catalogue of BmNPV genes will be a helpful reference in the functional analysis of viral genes.