The neutralizing human recombinant antibodies to pathogenic Orthopoxviruses derived from a phage display immune library

► A panel of recombinant human antibodies to orthopoxviruses was developed. ► Seven phage-displaying scFvs neutralized CPXV and VACV in PRNT. ► Five phage-displaying scFvs neutralize Monkeypox virus (MPXV) in PRNT. ► Neutralizing antibodies recognized recombinant J3L protein in Western blot analysis. ► Fully human neutralizing mAb displayed Kd of 0.7 nM.