کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6143487 | 1228247 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Host genetic variation in susceptibility to Punta Toro virus
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PTVPunta Toro virusreverse transcriptase quantitative PCRBunyaviridaeRVFVs.c.RT-qPCRGAPDHLD50DPI50% lethal dose - 50٪ مرگ و میرdays post infection - روز پس از عفونتsubcutaneously - زیر جلدیPhlebovirus - فیبرو ویروسplaque-forming units - واحدهای پلاک سازیRift Valley fever virus - ویروس ریفت ویل درهpfu - پفوmurine - چینیglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Host genetic variation in susceptibility to Punta Toro virus Host genetic variation in susceptibility to Punta Toro virus](/preview/png/6143487.png)
چکیده انگلیسی
Infection of small laboratory animals by Punta Toro virus (PTV), family Bunyaviridae, genus Phlebovirus, is a model for the study of the human pathogen Rift Valley fever virus (RVFV). We have identified inbred mouse strains with significant differences in host response to the Adames strain of PTV. Nine inbred strains of mice representing major branches in the Mus musculus phylogeny were inoculated subcutaneously with a high dose of PTV in survival experiments. Two inbred strains of mice, NZW/LacJ and 129S1/SvImJ, died â¼4Â days after PTV infection, whereas 7 other strains survived the challenge and showed no clinical signs of disease. Histologically, 129S1/SvImJ mice showed massive hepatocellular necrosis and had additional lesions in lung, brain, and spleen, whereas NZW/LacJ mice had mild piecemeal hepatocellular necrosis. PTV viral loads in the livers of infected mice were determined by reverse transcriptase quantitative PCR. Inbred mice from strains that showed clinical signs and succumbed to PTV infection had higher liver viral loads than did mice of resistant strains. Hybrid F1 mice were generated by crossing susceptible 129S1 and resistant FVB/N mice and tested for susceptibility. The hybrid F1 mice showed significantly higher viral loads in the liver than the resistant parental FVB/N mice, suggesting that susceptibility is dominant. These findings will enable an unbiased genetic approach to identify host genes mediating susceptibility to PTV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 157, Issue 1, April 2011, Pages 71-75
Journal: Virus Research - Volume 157, Issue 1, April 2011, Pages 71-75
نویسندگان
Shanna L. Ashley, Stefanie M. Ameres, Sonja R. Gerrard, Oded Foreman, Kathryn A. Eaton, Jason B. Weinberg, Katherine R. Spindler,