کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6188321 | 1600700 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
COX-2 gene promoter DNA methylation status in eutopic and ectopic endometrium of Egyptian women with endometriosis
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کلمات کلیدی
CRECOX-2NF-kBPGSNF-IL6MS-PCRmethylation specific polymerase chain reactionEndometriosis - اندومتریوزEpigenetics - اپی ژنتیکDNA hypomethylation - دی ان ای متیلسیون DNACyclooxygenase-2 - سیکلوکوکسیژناز2cyclic AMP response element - عنصر پاسخ AMP cyclicnuclear factor-kappa beta - فاکتور هسته ای - کاپا بتاProstaglandins - پروستاگلاندین ها
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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چکیده انگلیسی
The pathophysiology of COX-2 expression in endometriosis is a matter of debate. The aim was to investigate the role of DNA methylation of the NF-IL6 site within the promoter of COX-2 gene in the pathogenesis of endometriosis. The endometrial tissues (ectopic and eutopic) were collected from 60 women with endometriosis and 30 women without endometriosis (control group). The methylation status of COX-2 was examined by methylation-specific PCR. Quantitative real-time PCR (RT-PCR) was performed to measure COX-2 mRNA levels in endometrial tissues. We found significantly higher levels of COX-2 in ectopic endometriotic tissue compared with eutopic tissue. Also, we found that the frequencies of methylation status of the NF-IL6 site within the COX-2 promoter in the eutopic and ectopic endometrial tissues of endometriosis groups were significantly decreased in comparison to controls (PÂ =Â 0.002, PÂ =Â 0.000 respectively). Our study demonstrated that DNA hypomethylation of the NF-IL6 site within the promoter of COX-2 gene could be a key mechanism for its elevated expression in the eutopic and ectopic tissues of endometriosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Reproductive Immunology - Volume 112, November 2015, Pages 63-67
Journal: Journal of Reproductive Immunology - Volume 112, November 2015, Pages 63-67
نویسندگان
Haidy E. Zidan, Noha A. Rezk, Amr Abd Almohsen Alnemr, Amany M. Abd el Ghany,