کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6197271 1602609 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simvastatin induces caspase-dependent apoptosis and activates P53 in OCM-1 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
Simvastatin induces caspase-dependent apoptosis and activates P53 in OCM-1 cells
چکیده انگلیسی


- Simvastatin reduces the viability of OCM-1 cells.
- Simvastatin inhibits proliferation and induces G1-phase arrest in OCM-1 cells.
- Simvastatin induced apoptosis in OCM-1 cells via a mitochondrial-dependent pathway.
- The mevalonate pathway may be a new therapeutic target of human choroidal melanoma.

Simvastatin is a cholesterol-lowering drug which exhibits numerous pleiotropic effects including anti-cancer activity. Yet, the anti-cancer effects in choroidal melanoma remain poorly characterized. Therefore, in this study, we investigated the effects of simvastatin on OCM-1 cells growth, apoptosis and cycle. Simvastatin showed an inhibitory effects on OCM-1 cells viability in dose-dependent (2-10 μM) and time-dependent (24-72 h) manner. Further study suggested that simvastatin-induced inhibition OCM-1 cells proliferation was associated with G1 phase arrest, decreased protein and mRNA expression of proliferation marker cyclin D1, cyclin E, cyclin dependent kinase (CDK)2 and increased expression of CDK inhibitory protein P21. In addition, simvastatin resulted in an increase in levels of reactive oxygen species (ROS) in OCM-1 cells and simvastatin significantly triggered apoptosis in OCM-1 cells, which was characterized by increased chromatin condensation, activation of caspase-9 and cleaved-caspase-3, increased expression mitochondrion-related apoptosis protein of P53, Bax and decreased expression of Bcl2 and iASPP. Collectively, our study demonstrated that simvastatin can efficiently inhibit proliferation and induce apoptosis in OCM-1 cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 113, August 2013, Pages 128-134
نویسندگان
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