کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6240370 | 1280421 | 2016 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A small molecule neutrophil elastase inhibitor, KRP-109, inhibits cystic fibrosis mucin degradation A small molecule neutrophil elastase inhibitor, KRP-109, inhibits cystic fibrosis mucin degradation](/preview/png/6240370.png)
BackgroundNeutrophil elastase (NE) rapidly degrades gel-forming airway mucins in cystic fibrosis (CF) sputum. We hypothesized that KRP-109, a small molecule NE inhibitor, would inhibit CF mucin degradation in vitro.MethodsSputa were collected from CF patients (n = 5) chronically or intermittently infected with Pseudomonas aeruginosa (P.a.). Mucin degradation was analyzed using western blot. Protease inhibitor studies were performed using alpha1-proteinase inhibitor (A1-PI Prolastin®) and KRP-109. Elastase activity assays were performed using spectrophotometry.ResultsThere were significant differences in the amount of active NE in different CF sputum samples. KRP-109 decreased the NE driven mucin degradation in vitro. Pseudomonas elastases appeared to blunt elastase inhibition by A1-PI or KRP-109.ConclusionInhibitors of neutrophil and Pseudomonas-derived elastases might rescue mucus clearance and reverse airway obstruction in CF.
Journal: Journal of Cystic Fibrosis - Volume 15, Issue 3, May 2016, Pages 325-331