کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6262962 1613817 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research ReportMelatonin, minocycline and ascorbic acid reduce oxidative stress and viral titers and increase survival rate in experimental Venezuelan equine encephalitis
ترجمه فارسی عنوان
گزارش تحقیقاتی ملاتونین، مینوسیکلین و اسید آسکوربیک باعث کاهش استرس اکسیداتیو و تیترهای ویروسی و افزایش میزان بقای آن در انسفالیت عضوی آزمایشی ونزوئلا
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


- VEE virus replicates and induces oxidative stress in mouse brain.
- VEE virus replicates and induces oxidative stress in neuroblastoma cell cultures.
- Melatonin, minocycline and ascorbic acid reduce those parameters.
- Those compounds have potential therapeutic use in VEE infection.

Venezuelan equine encephalitis (VEE) virus causes an acute central nervous system infection in human and animals. Melatonin (MLT), minocycline (MIN) and ascorbic acid (AA) have been shown to have antiviral activities in experimental infections; however, the mechanisms involved are poorly studied. Therefore, the aim of this study was to determine the effects of those compounds on the viral titers, NO production and lipid peroxidation in the brain of mice and neuroblastoma cultures infected by VEE virus. Infected mouse (10 LD50) were treated with MLT (500 μg/kg bw), MIN (50 mg/kg bw) or AA (50 mg/kg bw). Infected neuroblastoma cultures (MOI: 1); MLT: 0.5, 1, 5 mM, MIN: 0.1, 0.2, 2 μM or AA: 25, 50, 75 μM. Brains were obtained at days 1, 3 and 5. In addition, survival rate of infected treated mice was also analyzed. Viral replication was determined by the plaque formation technique. NO and lipid peroxidation were measured by Griess׳ reaction and thiobarbituric acid assay respectively. Increased viral replication, NO production and lipid peroxidation were observed in both, infected brain and neuroblastoma cell cultures compared with uninfected controls. Those effects were diminished by the studied treatments. In addition, increased survival rate (50%) in treated infected animals compared with untreated infected mice (0%) was found. MLT, MIN and AA have an antiviral effect involving their anti-oxidant properties, and suggesting a potential use of these compounds for human VEE virus infection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1622, 5 October 2015, Pages 368-376
نویسندگان
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