کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6263128 | 1613831 | 2015 | 12 صفحه PDF | دانلود رایگان |
- RSV prevents ischemia-induced depletion of GLT-1 expression in the CA1 region.
- GLT-1 expression is positively correlated with hippocampal CA1 neuronal density.
- Effects of RSV on microglia activation in vulnerable CA1 neurons are dose-dependent.
- RSV normalizes CA1-GFAP to control levels following global cerebral ischemia.
The naturally occurring polyphenol phytoalexin resveratrol (RSV) regulates neuronal inflammation in various disease models and protects the brain against ischemic injury. Cell surface glutamate transporters on perisynaptic astrocytes are important regulators of extracellular glutamate levels and synaptic activation. Following cerebral ischemia, reduced astroglial type-1 glutamate transporter (GLT-1) expression in the CA1 pyramidal layers of the hippocampus contribute to neurotoxic glutamate levels. The current study examined the effects of 21-day RSV pretreatment (1 or 10Â mg/kg dose; i.p.) on microglia and astrocyte activation and characterized GLT-1 expression in the dentate gyrus (DG), CA1 and CA3 layers of the hippocampus 7 days following 10Â min global ischemia. Male Wistar rats were divided into five groups; sham/saline, ischemia/saline, ischemia/1Â mg/kg RSV, ischemia/10Â mg/kg RSV and sham/10Â mg/kg RSV. Immunohistochemical detection of GLT-1, CD11b/c, glial fibrillary acidic protein (GFAP) assessed type 1 glutamate transporter expression and microglial/glial cell activation following sham surgery or global ischemia. Our findings demonstrate prevention by RSV of ischemia-induced reduction of GLT-1 expression in the vulnerable CA1 layer 7 days following global ischemia, which was accompanied by the polyphenol's inhibition of post ischemic increase in CD11b/c and GFAP expression. RSV also conferred significant CA1 neuronal protection positively correlated with attenuation of glutamate transporter activation. These findings support beneficial effects of RSV in modulation of the excitotoxic cascade postischemia, which are congruent with anti-inflammatory effects observed in various pathological models.
Journal: Brain Research - Volume 1608, 22 May 2015, Pages 203-214