Advances in thin tissue Golgi-Cox impregnation: Fast, reliable methods for multi-assay analyses in rodent and non-human primate brain

► We describe thin tissue 'Golgi-Cox' methods that allow fast impregnation without significant crystallization artifact. ► These methods are compatible with the tissue fixatives acrolein, glutaraldehyde, or paraformaldehyde. ► Fixative perfusion allows using alternate sections for analyses such as immunohistochemistry and electron microscopy. ► We also describe immunohistochemistry and Golgi impregnation in the same tissue section without de-impregnation protocols. ► We detail advantages and disadvantages to guide for the investigator interested in tailoring Golgi-Cox for their own use.