A method for stable transgenesis of radial glia lineage in rat neocortex by piggyBac mediated transposition

► A lineage labeling method using piggyBac transposon mediated transgenesis. ► A piggyBac plasmid toolkit for gain-of-function and loss-of-function approaches. ► Labeling subpopulations of neural progenitors and progeny by pairing promoters in donor and helper plasmids. ► Stable transgenesis by in utero electroporation of astrocytes, oligodendrocytes and olfactory bulb interneurons.