A HaloTag® method for assessing the retrograde axonal transport of the p75 neurotrophin receptor and other proteins in compartmented cultures of rat sympathetic neurons

► We describe a method for studying the axonal transport of endogenous proteins fused to HaloTag® in rat sympathetic neurons in compartmented cultures. ► Applying HaloTag ligand to the distal axons allows the tracking of labeled proteins during retrograde transport. ► We demonstrate that the transport of HaloTag protein alone does not interfere with tracking the transport of HaloTag fusion proteins. ► We demonstrate the retrograde transport of HaloTag protein fused to the p75 neurotrophin receptor. ► We also demonstrate the anterograde transport of HaloTag protein fused to tubulin.