کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6270005 1295172 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Imaging fluid flow and cilia beating pattern in Xenopus brain ventricles
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Imaging fluid flow and cilia beating pattern in Xenopus brain ventricles
چکیده انگلیسی

Brain development and health depends upon the efficient movement of the cerebrospinal fluid inside of brain ventricles. When disrupted either through mutation, disease, or physiological damage, brain function becomes significantly impaired. Here I present a simple method of following cerebrospinal fluid circulation in Xenopus tadpoles using fluorescent microspheres which can be applied to imaging fluid circulation in any transparent embryo. In particular, cilia may be labeled with these microspheres to study their dynamics and movement patterns in vivo while simultaneously measuring bulk fluid flow. This technique will facilitate the analysis of fluid dynamics in developing embryos and aid in understanding the regulation of cilia dependent fluid flow in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 189, Issue 1, 30 May 2010, Pages 1-4
نویسندگان
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