کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6271603 | 1614766 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inflammatory mediator-induced modulation of GABAA currents in human sensory neurons
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کلمات کلیدی
DRGpKaPKCdorsal root ganglion - گانگلیون ریشه پشتیTHIP - آنTyrosine kinase - تیروزین کینازCNS - دستگاه عصبی مرکزیcentral nervous system - سیستم عصبی مرکزیintracellular Cl− concentration - غلظت کلسیم داخل سلولیprotein kinase A - پروتئین کیناز AProtein kinase C - پروتئین کیناز سیPatch clamp - پچ گیرGABA - گاباGABAA receptor - گیرنده GABAA یا گیرنده گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Inflammatory mediator-induced modulation of GABAA currents in human sensory neurons Inflammatory mediator-induced modulation of GABAA currents in human sensory neurons](/preview/png/6271603.png)
چکیده انگلیسی
The purpose of the present study was to characterize the properties of A-type GABA receptor (GABAA receptor) currents in human sensory neurons. Neurons were obtained from adult organ donors. GABAA currents were recorded in isolated neurons. Both large inactivating low-affinity currents and smaller persistent high-affinity currents were present in all of the 129 neurons studied from 15 donors. The kinetics of human GABAA currents were slower than those in rat sensory neurons. GABA currents were completely blocked by bicuculline (10 μM), and persistent currents were activated by the δ-subunit-preferring agonist, 4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridine-3-ol (THIP). The GABA current equilibrium potential was â¼20 mV more hyperpolarized than in rat neurons. Both low- and high-affinity currents were increased by inflammatory mediators but via different second messenger pathways. These results highlight potentially important species differences in the properties of ion channels present in their native environment and suggest the use of human sensory neurons may be a valuable tool to test compounds prior to use in humans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 310, 3 December 2015, Pages 401-409
Journal: Neuroscience - Volume 310, 3 December 2015, Pages 401-409
نویسندگان
X.-L. Zhang, K.-Y. Lee, B.T. Priest, I. Belfer, M.S. Gold,