کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6271676 | 1614765 | 2015 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Baicalin decreases SGK1 expression in the hippocampus and reverses depressive-like behaviors induced by corticosterone Baicalin decreases SGK1 expression in the hippocampus and reverses depressive-like behaviors induced by corticosterone](/preview/png/6271676.png)
- Baicalin prevents depressive-like behaviors induced by CORT.
- Baicalin reverses the increased mRNA and protein expression of SGK1 in the hippocampus induced by CORT.
- Baicalin increases the hippocampal protein expression of 11β-HSD2, GRα and BDNF in CORT-treated mice.
The present study was to investigate whether baicalin can prevent repeated exogenous corticosterone injection-induced depressive-like behaviors and explore its possible mechanisms. After a 21-day treatment with baicalin (10 and 20 mg/kg), sucrose preference in the sucrose preference test (SPT) and immobility time in forced swimming test (FST) were observed, serum corticosterone levels and brain-derived neurotrophic factor (BDNF) contents in the hippocampus were examined by enzyme-linked immunosorbent assay (ELISA). In addition, quantitative real-time polymerase chain reaction (qPCR) and western blot were used to detect the mRNA and protein expression in the hippocampus. The results showed that 21-day cortiscosterone injections caused depressive-like behaviors in mice, including the reduced sucrose preference and increased duration of immobility. Baicalin reversed these behavioral changes described above and restored serum corticosterone levels. Additionally, baicalin up-regulated the mRNA and protein expression of glucocorticoid receptor (GR) and BDNF, accompanied with the down-regulation of serum- and glucocorticoid-regulated kinase 1 (SGK1) in the hippocampus. Moreover, baicalin significantly increased the protein expression of 11β-hydroxysteroid dehydrogenase-2 (11β-HSD2) in the hippocampus. The present results confirmed the antidepressant-like effects of baicalin in a mice model of depression induced by corticosterone and suggested that its mechanism was possibly involved in reducing serum corticosterone and thereby increasing BDNF in the hippocampus.
Journal: Neuroscience - Volume 311, 17 December 2015, Pages 130-137