کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6273025 | 1614792 | 2015 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inflammation-induced increase in nicotinic acetylcholine receptor current in cutaneous nociceptive DRG neurons from the adult rat
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کلمات کلیدی
MLACFAIB4TRPA1DRGAITCnAChREGTAHEPESMECdorsal root ganglion - گانگلیون ریشه پشتی1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate - 1،1'-dioctadecyl-3،3،3 '، 3'-tetramethylindocarbocyanine perchlorate4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acidcomplete Freund’s adjuvant - adjuvant دوست کاملDiI - DIIDMSO - DMSOethylene glycol tetraacetic acid - اتیلن گلیکول تتراستیک اسیدAllyl isothiocyanate - ایزوتیوسیانات آللییلisolectin B4 - ایزوکتین B4analysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceNociceptor sensitization - حساسیت زخم معدهInflammatory pain - درد التهابیDimethyl sulfoxide - دیمتیل سولفواکسیدmethyllycaconitine citrate - متیل لیکاکونیتین سیتراتMecamylamine - مکامیلامینHex - هگزاHexamethonium - هگزامتونیVoltage-clamp - ولتاژ گیرligand-gated ion channel - کانال یون لیگاند دارCaP - کلاه لبه دارCapsaicin - کپسایسین یا کاپسیسینnicotinic acetylcholine receptor - گیرنده استیلکولین نیکوتینCurrent clamp - گیره کنونی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The goals of the present study were to determine (1) the properties of the nicotinic acetylcholine receptor (nAChR) currents in rat cutaneous dorsal root ganglion (DRG) neurons; (2) the impact of nAChR activation on the excitability of cutaneous DRG neurons; and (3) the impact of inflammation on the density and distribution of nAChR currents among cutaneous DRG neurons. Whole-cell patch-clamp techniques were used to study retrogradely labeled DRG neurons from naïve and complete Freund's adjuvant inflamed rats. Nicotine-evoked currents were detectable in â¼70% of the cutaneous DRG neurons, where only one of two current types, fast or slow currents based on rates of activation and inactivation, was present in each neuron. The biophysical and pharmacological properties of the fast current were consistent with nAChRs containing an α7 subunit while those of the slow current were consistent with nAChRs containing α3/β4 subunits. The majority of small diameter neurons with fast current were IB4â while the majority of small diameter neurons with slow current were IB4+. Preincubation with nicotine (1 μM) produced a transient (1 min) depolarization and increase in the excitability of neurons with fast current and a decrease in the amplitude of capsaicin-evoked current in neurons with slow current. Inflammation increased the current density of both slow and fast currents in small diameter neurons and increased the percentage of neurons with the fast current. With the relatively selective distribution of nAChR currents in putative nociceptive cutaneous DRG neurons, our results suggest that the role of these receptors in inflammatory hyperalgesia is likely to be complex and dependent on the concentration and timing of acetylcholine release in the periphery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 284, 22 January 2015, Pages 483-499
Journal: Neuroscience - Volume 284, 22 January 2015, Pages 483-499
نویسندگان
X.-L. Zhang, K.M. Albers, M.S. Gold,