کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6273123 | 1614790 | 2015 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A role for the outer retina in development of the intrinsic pupillary light reflex in mice
ترجمه فارسی عنوان
نقش شبکیه بیرونی در رشد رفلکس نور ذاتی در موش
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کلمات کلیدی
rd miceLCMDBrn3bTBPRGCCMZipRGCBRN3Aciliary marginal zonePLRRetinitis pigmentosaOPNanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceintrinsically photosensitive retinal ganglion cell - درون سلول گانگلیونی شبکیه حساس به نور درونی استDevelopment - رشدpupillary light reflex - رفلکس نورسنجیPigmentation - رنگدانهretinal ganglion cell - سلول گانگلیونی شبکیهlaser capture microdissection - لیزر ضبط میکرو دیسکسیونmelanopsin - ملانپسینolivary pretectal nucleus - هسته pretectal olivaryquantitative real time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPCR - واکنش زنجیرهٔ پلیمرازTATA-binding protein - پروتئین متصل به TATAPostnatal - پس از زایمان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
چکیده انگلیسی
Mice do not require the brain in order to maintain constricted pupils. However, little is known about this intrinsic pupillary light reflex (iPLR) beyond a requirement for melanopsin in the iris and an intact retinal ciliary marginal zone (CMZ). Here, we study the mouse iPLR in vitro and examine a potential role for outer retina (rods and cones) in this response. In wild-type mice the iPLR was absent at postnatal day 17 (P17), developing progressively from P21-P49. However, the iPLR only achieved â¼30% of the wild-type constriction in adult mice with severe outer retinal degeneration (rd and rdcl). Paradoxically, the iPLR increased significantly in retinal degenerate mice >1.5Â years of age. This was accompanied by an increase in baseline pupil tone in the dark to levels indistinguishable from those in adult wild types. This rejuvenated iPLR response was slowed by atropine application, suggesting the involvement of cholinergic neurotransmission. We could find no evidence of an increase in melanopsin expression by quantitative PCR in the iris and ciliary body of aged retinal degenerates and a detailed anatomical analysis revealed a significant decline in melanopsin-positive intrinsically photosensitive retinal ganglion cells (ipRGCs) in rdcl mice >1.5Â years. Adult mice lacking rod function (Gnat1â/â) also had a weak iPLR, while mice lacking functional cones (Cpfl5) maintained a robust response. We also identify an important role for pigmentation in the development of the mouse iPLR, with only a weak and transient response present in albino animals. Our results show that the iPLR in mice develops unexpectedly late and are consistent with a role for rods and pigmentation in the development of this response in mice. The enhancement of the iPLR in aged degenerate mice was extremely surprising but may have relevance to behavioral observations in mice and patients with retinitis pigmentosa.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 286, 12 February 2015, Pages 60-78
Journal: Neuroscience - Volume 286, 12 February 2015, Pages 60-78
نویسندگان
A. Vugler, M. Semo, A. OrtÃn-MartÃnez, A. Rojanasakul, B. Nommiste, F.J. Valiente-Soriano, D. GarcÃa-Ayuso, P. Coffey, M. Vidal-Sanz, C. Gias,