کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6273142 | 1614791 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Localization of aquaporin 1 water channel in the Schmidt-Lanterman incisures and the paranodal regions of the rat sciatic nerve
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کلمات کلیدی
DABPBS-ASLIAQPTBSTRT-PCREGTADRGGFAPSDSMBP3,3′-diaminobenzidine - 3،3'-diaminobenzidineTris-buffered saline with Tween 20 - Tris-buffered saline با Tween 20aquaporin - آکواپورینEDTA - اتیلن دی آمین تترا استیک اسید ethylene glycol tetraacetic acid - اتیلن گلیکول تتراستیک اسیدEthylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدsodium dodecyl sulfate - سدیم دودسیل سولفاتSchwann cells - سلول های Schwannperipheral nervous system - سیستم عصبی پیرامونیMyelin - میلینpolymerase chain reaction - واکنش زنجیره ای پلیمرازreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسPCR - واکنش زنجیرهٔ پلیمرازGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالMyelin basic protein - پروتئین پایه میلینPNS - کارمندان دولتdorsal root ganglia - گانگلیس ریشه پشتیNodes of Ranvier - گره های Ranvier
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Aquaporin 1 (AQP1) is a member of a family of small, integral membrane water-transporting proteins, which facilitate water movement across cell membranes in response to osmotic gradients. Several papers have studied the expression and function of the AQPs in the central nervous system. However, little is known about the AQPs in the peripheral nervous system (PNS). In the PNS, AQP1, AQP2 and AQP4 have been reported in both peripheral neurons and glial cells. In this work we studied the expression and localization of AQP1 in the rat sciatic nerve. We found that from the four AQPs we studied (AQP1, AQP2, AQP4 and AQP9) only AQP1 is expressed in the nerve by reverse transcription polymerase chain reaction (RT-PCR). AQP1 is also observed at the protein level by Western blot analysis. We also studied the localization of AQP1 in the sciatic nerve by immunohistochemistry. The results show that AQP1 is present in both myelinating and non-myelinating Schwann cells. In myelin internodes AQP1 is enriched in the Schmidt-Lanterman incisures and in some internodes it is also present in the abaxonal membrane. At the nodes of Ranvier, AQP1 co-localizes with actin in the paranodal regions of the nerve. Therefore, AQP1 might play an important role in myelin homeostasis maintaining the thermodynamic equilibrium across the plasma membrane in myelinated axons during electrical activity. Also the expression of AQP1 in non-myelinating Schwann cells supports the involvement of AQP1 in pain perception.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 285, 29 January 2015, Pages 119-127
Journal: Neuroscience - Volume 285, 29 January 2015, Pages 119-127
نویسندگان
E. Segura-Anaya, A. MartÃnez-Gómez, M.A.R. Dent,