کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6273573 | 1614799 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Common effects of fat, ethanol, and nicotine on enkephalin in discrete areas of the brain
ترجمه فارسی عنوان
اثرات متداول چربی، اتانول و نیکوتین بر روی آنکفالین در مناطق گسسته مغز
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کلمات کلیدی
sodium chloride and sodium citrateNAcShNAc CoreBLAAMYGNACNAccSSCS.E.M.EPMCeA - CEAAmygdala - آمیگدال، بادامهγ-aminobutyric acid - اسید γ-آمینوبوتیریکEnk - انکenkephalin - انکافالینImmunoreactivity - ایمنی فعالphosphate buffer - بافر فسفاتelevated plus maze - بالا به همراه پیچ و خمTriglycerides - تریگلیسریدstandard error of the mean - خطای استاندارد میانگینdigoxigenin - دیگوکسین ژنSucrose - ساکارزDIG - شماfluorescence in situ hybridization - فلورسانس در هیبریداسیون در محلPVN - مالیات بر ارزش افزودهFish - ماهیRat - موش صحراییNAc Shell - ناس شلNucleus accumbens - هسته accumbenscentral nucleus of the amygdala - هسته مرکزی آمیگدالbasolateral nucleus of the amygdala - هسته نزولی amygdalaparaventricular nucleus of the hypothalamus - هسته پروانه مرکزی هیپوتالاموسhypo - هیپوHypothalamus - هیپوتالاموسCORT - کورتCorticosterone - کورتیکوسترونGABA - گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
چکیده انگلیسی
Fat, ethanol, and nicotine share a number of properties, including their ability to reinforce behavior and produce overconsumption. To test whether these substances act similarly on the same neuronal populations in specific brain areas mediating these behaviors, we administered the substances short-term, using the same methods and within the same experiment, and measured their effects, in areas of the hypothalamus (HYPO), amygdala (AMYG), and nucleus accumbens (NAc), on mRNA levels of the opioid peptide, enkephalin (ENK), using in situ hybridization and on c-Fos immunoreactivity (ir) to indicate neuronal activity, using immunofluorescence histochemistry. In addition, we examined for comparison another reinforcing substance, sucrose, and also took measurements of stress-related behaviors and circulating corticosterone (CORT) and triglycerides (TG), to determine if they contribute to these substances' behavioral and physiological effects. Adult Sprague-Dawley rats were gavaged three times daily over 5Â days with 3.5Â mL of water, Intralipid (20% v/v), ethanol (12% v/v), nicotine (0.01% w/v) or sucrose (22% w/v) (approximately 7Â kcal/dose), and tail vein blood was collected for measurements of circulating CORT and TG. On day five, animals were sacrificed, brains removed, and the HYPO, AMYG, and NAc processed for single- or double-labeling of ENK mRNA and c-Fos-ir. Fat, ethanol, and nicotine, but not sucrose, increased the single- and double-labeling of ENK and c-Fos-ir in precisely the same brain areas, the middle parvocellular but not lateral area of the paraventricular nucleus, central but not basolateral nucleus of the AMYG, and core but not shell of the NAc. While having little effect on stress-related behaviors or CORT levels, fat, ethanol, and nicotine all increased circulating levels of TG. These findings suggest that the overconsumption of these three substances and their potential for abuse are mediated by the same populations of ENK-expressing neurons in specific nuclei of the hypothalamus and limbic system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 277, 26 September 2014, Pages 665-678
Journal: Neuroscience - Volume 277, 26 September 2014, Pages 665-678
نویسندگان
G.-Q. Chang, O. Karatayev, J.R. Barson, S.C. Liang, S.F. Leibowitz,