کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6276781 | 1295742 | 2010 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of caspase-3-mediated apoptosis improves spinal cord repair in a regeneration-competent vertebrate system
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کلمات کلیدی
ethyl 3-aminobenzoate methanesulfonateEODPBSMS-222DAPITBS5-bromo-2′-deoxyuridine - 5-bromo-2'-deoxyuridineBSA - BSADMSO - DMSOSpinal cord injury - آسیب نخاعیbovine serum albumin - آلبومین سرم گاوSpinal cord regeneration - بازسازی نخاعBrdU - بروموداکسی اوریدینanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceelectric organ discharge - تخلیه عضو بدنTris-buffered saline - تریس بافر شورApoptosis - خزان یاختهایRoom temperature - دمای اتاقDimethyl sulfoxide - دیمتیل سولفواکسیدsci - علمیNeuroprotection - محافظت نورونی یا محافظت از عصبPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCaspase-3 - کاسپاز ۳
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Teleost fish exhibit an excellent potential for structural and functional recovery after CNS lesions. The function of apoptosis in the process of regeneration remains controversial. While some studies have identified this type of cell death as essential for successful regeneration, other investigations have suggested some degree of functional improvement after inhibition of apoptosis. In the present study, we examined whether inhibition of apoptosis immediately after injury can improve spinal cord regeneration. As a model system, we used Apteronotus leptorhynchus, a regeneration-competent weakly electric fish. To inhibit apoptosis, we employed 2,2â²-methylenebis (1,3-cyclohexanedione) (M50054), a compound that prevents caspase-3 activation. Administration of this apoptosis inhibitor led to a significant reduction in the numbers of apoptotic cells at 24 h, 5 days, and 30 days after the lesion. Using triple immunolabeling, we identified a significant reduction in the level of apoptosis at 5 and 30 days after the lesion among the following cellular categories: cells generated shortly after the lesion, existing neurons, and newly differentiated neurons. This reduced rate of apoptosis led to an increase in the relative number of differentiating and surviving neurons at both 5 and 30 days post-injury, compared to the control groups. Functional regeneration, as indicated by the recovery rate of the amplitude of the electric organ discharge (EOD), was significantly improved within the first 20 days after the lesion in the fish treated with M50054. Our data provide the first evidence that modulation of caspase-3 activation can significantly improve neuroregeneration and functional recovery in a regeneration-competent organism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 171, Issue 2, 1 December 2010, Pages 599-612
Journal: Neuroscience - Volume 171, Issue 2, 1 December 2010, Pages 599-612
نویسندگان
R.F. Sîrbulescu, G.K.H. Zupanc,