کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277267 | 1295754 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Stress hormone synthesis in mouse hypothalamus and adrenal gland triggered by restraint is dependent on pituitary adenylate cyclase-activating polypeptide signaling
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کلمات کلیدی
FBJ osteosarcoma oncogenePNMTFOSEGR1IEGCRHmRNAPACAPqRT-PCRmessenger RNA - RNA messengeranalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancetyrosine hydroxylase - تیروزین هیدروکسیلازRestraint - خویشتن داریPhenylethanolamine N-methyltransferase - فنیلتانولامین N-متیل ترانسفرازPVN - مالیات بر ارزش افزودهHPA axis - محور هیپوتالاموس-هیپوفیز-آدرنالHypothalamic-pituitary-adrenocortical axis - محور هیپوتالاموس-هیپوفیز-آدرنوکورتیکNeuropeptide - نوروپپتیدparaventricular nucleus of the hypothalamus - هسته پروانه مرکزی هیپوتالاموسcorticotropin-releasing hormone - هورمون آزاد کننده کورتیکوتروپینquantitative reverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی معکوس و پلیمریزا معکوسpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازearly growth response 1 - پاسخ رشد اولیه 1pituitary adenylate cyclase-activating polypeptide - پلیپپتید فعال آدنیلات سیکلاس هیپوفیزImmediate-early gene - ژن سریع و سریع
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Stress responses are elicited by a variety of stimuli and are aimed at counteracting direct or perceived threats to the well-being of an organism. In the mammalian central and peripheral nervous systems, specific cell groups constitute signaling circuits that indicate the presence of a stressor and elaborate an adequate response. Pituitary adenylate cyclase-activating polypeptide (PACAP) is expressed in central and peripheral parts of these circuits and has recently been identified as a candidate for regulation of the stress axis. In the present experiments, we tested the involvement of PACAP in the response to a psychological stressor in vivo. We used a restraint paradigm and compared PACAP-deficient mice (PACAPâ/â) to wild-type controls (PACAP+/+). Acute secretion of corticosterone elicited by 1 h of restraint was found to be identical between genotypes, whereas sustained secretion provoked by 6 h of unrelieved restraint was 48% lower in PACAPâ/âmice. Within the latter time frame, expression of messenger RNA (mRNA) encoding corticotropin-releasing hormone (CRH) was increased in the hypothalamus of wild type, but not PACAP-deficient mice. Expression of the activity-regulated transcription factors Egr1 (early growth response 1) and Fos (FBJ osteosarcoma oncogene) in the hypothalamus was rapidly and transiently induced by restraint in a PACAP-dependent fashion, a pattern that was also found in the adrenal glands. Here, abundance of transcripts encoding enzymes required for adrenomedullary catecholamine biosynthesis, namely TH (tyrosine hydroxylase) and PNMT (phenylethanolamine N-methyltransferase), was higher in PACAP+/+ mice after 6 h of unrelieved restraint. Our results suggest that sustained corticosterone secretion, synthesis of the hypophysiotropic hormone CRH in the hypothalamus, and synthesis of the enzymes producing the hormone adrenaline in the adrenal medulla, are controlled by PACAP signaling in the mouse. These findings identify PACAP as a major contributor to the stimulus-secretion-synthesis coupling that supports stress responses in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 165, Issue 4, 17 February 2010, Pages 1025-1030
Journal: Neuroscience - Volume 165, Issue 4, 17 February 2010, Pages 1025-1030
نویسندگان
N. Stroth, L.E. Eiden,