کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277437 | 1295757 | 2010 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Salicylate-induced degeneration of cochlea spiral ganglion neurons-apoptosis signaling
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کلمات کلیدی
inhibitor of caspase-activated DNaseNF-κBTNFFas-LICADBMEFADDRT-PCRDISCIAPOHCSGNReverse transcriptase PCR - PCR معکوس رونویسیcycle threshold - آستانه چرخهAspirin - آسپرینIHC - ایمونوهیستوشیمیsodium salicylate - سدیم سالیسیلاتouter hair cell - سلول بیرونی موInner hair cell - سلول داخلی داخلیCAD - طراحی به کمک رایانه یا کَدbasal medium Eagle - عقاب متوسطFas Ligand - فاس لیگاندtumor necrosis factor - فاکتور نکروز تومورOrganotypic culture - فرهنگ ارگانیکAuditory Cortex - قشر شنواییTRAIL - قطارTNF-related apoptosis-inducing ligand - لیگاند ناشی از آپوپتوز مرتبط با TNFinhibitor of apoptosis - مهارکننده آپوپتوزSpiral ganglion neurons - نورونهای گانگلیونی اسپیرالpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازCompound Action Potential - پتانسیل اقدام مشترکdeath-inducing signaling complex - پیچیدگی سیگنالینگ ناشی از مرگcaspase-activated DNase - کاسپاز فعال DNasecaspase - کسپاز یا کاسپازCaP - کلاه لبه دار
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Aspirin, whose active ingredient is sodium salicylate, is the most widely used drug worldwide, but it is not recommended for children because it may cause Reye's syndrome. High doses of salicylate also induce temporary hearing loss and tinnitus; while these disorders are believed to disappear when treatment is discontinued some data suggest that prolonged treatment may be neurotoxic. To investigate its ototoxicity, immature, postnatal day 3 rat cochlear organotypic cultures were treated with salicylate. Salicylate did not damage the sensory hair cells, but instead damaged the spiral ganglion neurons (SGN) and their peripheral fibers in a dose-dependent manner. The cross-sectional area of SGN decreased from 205 μm2 in controls to 143, 116, and 91 μm2 in cultures treated with 1, 3, or 5 mM salicylate, respectively. Morphological changes and caspase upregulation were indicative of caspase-mediated apoptosis. A quantitative RT-PCR apoptosis array identified a subset of genes up- or down regulated by salicylate. Eight genes showed a biologically relevant change (P<0.05, â¥2 fold change) after 3 h treatment with salicylate; seven genes (Tp53, Birc3, Tnfrsf5, Casp7, Nfkb1, Fas, Lta, Tnfsf10) were upregulated and one gene (Pycard) was downregulated. After 6 h treatment, only one gene (Nol3) was upregulated and two genes were downregulated (Cideb and Lhx4) while after 12 h treatment, two genes (Il10, Gadd45a) were upregulated and 4 (Prok2, Card10, Ltbr, Dapk1) were downregulated. High doses of salicylate in a physiologically relevant range can induce caspase-mediated cell death in immature SGN; changes in the expression of apoptotic genes particularly among members of the tumor necrosis factor (TNF) family appear to play an important role in the degeneration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 168, Issue 1, 16 June 2010, Pages 288-299
Journal: Neuroscience - Volume 168, Issue 1, 16 June 2010, Pages 288-299
نویسندگان
L. Wei, D. Ding, R. Salvi,