کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6278176 | 1295800 | 2009 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Numb-mediated neurite outgrowth is isoform-dependent, and requires activation of voltage-dependent calcium channels
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DMEMSPTBPTBPC12trkAVGCCPRRNGFSSISSAICAFBSCa2+ current - Ca2 + جریانDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoV1/2 - V1 / 2Numb - بی حسDifferentiation - تفکیکRTK, Receptor tyrosine kinase - تیروزین کینازهای گیرنده ایfetal bovine serum - سرم جنین گاوnerve growth factor - فاکتور رشد عصبphosphotyrosine-binding - فسفاتیزین اتصالproline-rich region - منطقه غنی پرولینNeurite - نوریتvoltage-gated calcium channel - کانال کلسیم با ولتاژ
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Numb-mediated neurite outgrowth is isoform-dependent, and requires activation of voltage-dependent calcium channels Numb-mediated neurite outgrowth is isoform-dependent, and requires activation of voltage-dependent calcium channels](/preview/png/6278176.png)
چکیده انگلیسی
Numb is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells by unknown mechanisms. Here we report that the neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator. In contrast, tyrosine kinase inhibitor, genistein, and selective receptor tyrosine kinase (TrkA) inhibitor, K252α did not affect SPTB Numb-mediated neurite outgrowth. MAP kinase inhibitor, PD98059 partially reduced SPTB Numb-mediated neurite outgrowth. Cells expressing SPTB Numbs exhibit increased whole-cell Ca2+ current densities (ICa) which can be prevented by preincubation of either nifedipine or PD98095. Cells expressing LPTB Numbs expressed little ICa (density) and were not able to grow neurites. Our results indicate that Ca2+ influx through VGCC may be required for SPTB Numb-mediated neurite outgrowth, suggesting that Numb promotes neuronal differentiation by a mechanism involving PTB domain-specific regulation of Ca2+ influx and MAP kinase activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 161, Issue 2, 30 June 2009, Pages 403-412
Journal: Neuroscience - Volume 161, Issue 2, 30 June 2009, Pages 403-412
نویسندگان
C.B. Lu, W. Fu, X. Xu, M.P. Mattson,