کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6278521 | 1295823 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glutamate stimulates glutamate receptor interacting protein 1 degradation by ubiquitin-proteasome system to regulate surface expression of GluR2
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کلمات کلیدی
N-methyl-d-aspartateprotein interacting with C kinase 1glutamate receptor interacting protein 1GRIP1MG132PICK1GluR2GluRMDCNMDATTXSDSPSDEGTAHRP6-Cyano-7-nitroquinoxaline-2,3-dione - 6-Cyano-7-nitroquinoxaline-2،3-dionelong term depression - افسردگی بلند مدتBAPTA - بیایپیتیایtetrodotoxin - تترو دوتوکسین postsynaptic density - تراکم PostinapticDegradation - تنزلPDZ domain - دامنه PDZreverse transcription - رونویسی معکوسsodium dodecyl sulfate - سدیم دودسیل سولفاتCNQX - سیانکیوایکسubiquitin–proteasome system - سیستم ubiquitin-proteasomeLTD - محدودMonodansylcadaverine - مونودانسیل کاداورینHorseradish peroxidase - پراکسیداز هوررادیشPropidium iodide - پروتئین یدیدglutamate - گلوتاماتAMPA receptor - گیرنده AMPAGlutamate receptor - گیرنده گلوماتUPS - یو پی اس
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
The glutamate receptor interacting protein 1 (GRIP1) is a scaffolding protein in postsynaptic density (PSD), tethering AMPA receptors to other signaling proteins. Here we report that glutamate stimulation caused a rapid reduction in protein levels of GRIP1, but not that of glutamate receptor (GluR) 1, GluR2 and protein interacting with C kinase 1 (PICK1) in rat primary cortical neuron cultures. Down-regulation of GRIP1 by glutamate was blocked by carbobenzoxyl-leucinyl-leucinyl-leucinal (MG132), a proteasome inhibitor and by expression of K48R-ubiquitin, a dominant negative form of ubiquitin. The GRIP1 reduction was inhibited by MK-801, an N-methyl-d-aspartate (NMDA) receptor antagonist, but not by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), an AMPA receptor antagonist. EGTA and 1,2-bis(2-aminophenoxy)ethane-N,N,Nâ²,Nâ²-tetra acetic acid tetrakis (BAPTA), two Ca2+ chelators, but not nifedipine, an L-type Ca2+ channel blocker, prevented GRIP1 degradation. Furthermore, MG132 prevented glutamate-stimulated reduction in surface amount of GluR2, and knockdown of GRIP1 by RNAi against GRIP1 reduced surface GluR2 in neurons. Our results suggest that glutamate induces GRIP1 degradation by proteasome through an NMDA receptor-Ca2+ pathway and that GRIP1 degradation may play an important role in regulating GluR2 surface expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 145, Issue 1, 2 March 2007, Pages 100-109
Journal: Neuroscience - Volume 145, Issue 1, 2 March 2007, Pages 100-109
نویسندگان
L. Guo, Y. Wang,