کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6282192 | 1615133 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Voltage-gated sodium (NaV) channel blockade by plant cannabinoids does not confer anticonvulsant effects per se
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کلمات کلیدی
AEDCBDIFFPTZChlCBGVoltage-gated Na+ channelscannabigerol - canbabergolCho - برایEpilepsy - بیماری صرعChinese Hamster Ovary - تخمدان هامستر چینیseizure - تشنج antiepileptic drug - داروهای ضدصرعChinese hamster lung - ریه هامستر چینیDravet syndrome - سندرم DravetAnticonvulsant - ضد انعقادNav - نهPentylenetetrazole - پنتیلن تترازولCannabidiol - کانابیدیولCannabinoid - کانابینوئیدsodium channels - کانال های سدیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Voltage-gated sodium (NaV) channel blockade by plant cannabinoids does not confer anticonvulsant effects per se Voltage-gated sodium (NaV) channel blockade by plant cannabinoids does not confer anticonvulsant effects per se](/preview/png/6282192.png)
چکیده انگلیسی
Cannabidiol (CBD) is a non-psychoactive, well-tolerated, anticonvulsant plant cannabinoid, although its mechanism(s) of seizure suppression remains unknown. Here, we investigate the effect of CBD and the structurally similar cannabinoid, cannabigerol (CBG), on voltage-gated Na+ (NaV) channels, a common anti-epileptic drug target. CBG's anticonvulsant potential was also assessed in vivo. CBD effects on NaV channels were investigated using patch-clamp recordings from rat CA1 hippocampal neurons in brain slices, human SH-SY5Y (neuroblastoma) cells and mouse cortical neurons in culture. CBG effects were also assessed in SH-SY5Y cells and mouse cortical neurons. CBD and CBG effects on veratridine-stimulated human recombinant NaV1.1, 1.2 or 1.5 channels were assessed using a membrane potential-sensitive fluorescent dye high-throughput assay. The effect of CBG on pentyleneterazole-induced (PTZ) seizures was assessed in rat. CBD (10 μM) blocked NaV currents in SH-SY5Y cells, mouse cortical neurons and recombinant cell lines, and affected spike parameters in rat CA1 neurons; CBD also significantly decreased membrane resistance. CBG blocked NaV to a similar degree to CBD in both SH-SY5Y and mouse recordings, but had no effect (50-200 mg/kg) on PTZ-induced seizures in rat. CBD and CBG are NaV channel blockers at micromolar concentrations in human and murine neurons and recombinant cells. In contrast to previous reports investigating CBD, CBG had no effect upon PTZ-induced seizures in rat, indicating that NaV blockade per se does not correlate with anticonvulsant effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 566, 30 April 2014, Pages 269-274
Journal: Neuroscience Letters - Volume 566, 30 April 2014, Pages 269-274
نویسندگان
Andrew J. Hill, Nicholas A. Jones, Imogen Smith, Charlotte L. Hill, Claire M. Williams, Gary J. Stephens, Benjamin J. Whalley,