کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6282491 | 1615141 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Morphological analysis of embryonic cerebellar grafts in SCA2 mice
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کلمات کلیدی
eGFPSCAPCDPurkinje cell degenerationDNA - DNA یا اسید دزوکسی ریبونوکلئیکspinocerebellar ataxia - آتاکسی spinocerebellardeoxyribonucleic acid - اسید deoxyribonucleicCerebellar degeneration - دژنراسیون سلولPurkinje cells - سلول های PurkinjePurkinje cell - سلول پورکنژ، یاخته پورکینیهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استNeurotransplantation - پیوند عصبی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Morphological analysis of embryonic cerebellar grafts in SCA2 mice Morphological analysis of embryonic cerebellar grafts in SCA2 mice](/preview/png/6282491.png)
چکیده انگلیسی
SCA2 transgenic mice are thought to be a useful model of human spinocerebellar ataxia type 2. There is no effective therapy for cerebellar degenerative disorders, therefore neurotransplantation could offer hope. The aim of this work was to assess the survival and morphology of embryonic cerebellar grafts transplanted into the cerebellum of adult SCA2 mice. Four month-old homozygous SCA2 and negative control mice were treated with bilateral intracerebellar injections of an enhanced green fluorescent protein-positive embryonic cerebellar cell suspension. Graft survival and morphology were examined three months later. Graft-derived Purkinje cells and the presence of astrocytes in the graft were detected immunohistochemically. Nissl and hematoxylin-eosin techniques were used to visualize the histological structure of the graft and surrounding host tissue. Grafts survived in all experimental mice; no differences in graft structure, between SCA2 homozygous and negative mice, were found. The grafts contained numerous Purkinje cells but long distance graft-to-host axonal connections to the deep cerebellar nuclei were rarely seen. Relatively few astrocytes were found in the center of the graft. No signs of inflammation or tissue destruction were seen in the area around the grafts. Despite good graft survival and the presence of graft-derived Purkinje cells, the structure of the graft did not seem to promise any significant specific functional effects. We have shown that the graft is available for long-term experiments. Nevertheless, it would be beneficial to search for ways of enhancement of connections between the graft and host.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 558, 13 January 2014, Pages 154-158
Journal: Neuroscience Letters - Volume 558, 13 January 2014, Pages 154-158
نویسندگان
Zdenka Purkartova, Jan Tuma, Martin Pesta, Vlastimil Kulda, Lucie Hajkova, Ondrej Sebesta, Frantisek Vozeh, Jan Cendelin,