کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6282633 | 1296610 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Restoration of spatial memory dysfunction of human APP transgenic mice by transplantation of neuronal precursors derived from human iPS cells
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کلمات کلیدی
NFMMEFAβPDAPPAPPNCAMVGATMWMPBSiPS - IPSNOG - با این حالNeuron regeneration - بازسازی نورونembryoid bodies - جنین های جنینیvesicular GABA transporter - حامل ویسکوزر GABAShh - خیرRetinoic acid - رتینوئیک اسیدHuman iPS cells - سلول های انسانی انسانیdentate gyrus - شکنج دندانه دارsonic hedgehog - صدای جیر جیرPhosphate buffered saline - فسفات بافر شورFibronectin - فیبرونکتینMorris water maze - ماز آب آب موریسmouse embryonic fibroblasts - موش فیبروبلاست جنینیneural cell adhesion molecule - مولکول چسبندگی سلولی عصبیGABAergic neuron - نورون GABAergicCholinergic neuron - نورون چولینرژیکamyloid β protein - پروتئین β آمیلوئیدamyloid precursor protein - پروتئین پیش ماده آمیلوئیChAT - چتcholine acetyltransferase - کولین استیل ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
PDGF promoter driven amyloid precursor protein (PDAPP) transgenic mice were accompanied by age dependent amyloid β deposition and progressive spatial memory dysfunction which emerges within a few months of age. We conducted transplantation of neuronal precursors of cholinergic neuron phenotype which were derived from human iPS (hiPS) cells into bilateral hippocampus of PDAPP mice. We first generated neuronal precursors with cholinergic neuron phenotype from hiPS cells by culturing them with retinoic acid (RA), sonic hedgehog (SHH) and noggin-Fc (NOG). Spatial memory function of PDAPP mice was significantly impaired compared to that of nontransgenic littermates at age 8 weeks. After neuronal precursor transplantation, subsequent memory dysfunction of PDAPP mice was significantly improved, compared to that of vehicle injected PDAPP mice. We observed choline acetyltransferase (ChAT) positive cholinergic human neurons and vesicle GABA transporter (VGAT) positive GABAergic human neurons in PDAPP mouse hippocampus 45 days after the transplantation. Neuronal precursors with cholinergic neuron phenotype derived from hiPS cells survived in PDAPP mouse hippocampus and their spatial memory loss was improved. hiPS cells may become applicable for the treatment of patients with dementia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 557, Part B, 17 December 2013, Pages 129-134
Journal: Neuroscience Letters - Volume 557, Part B, 17 December 2013, Pages 129-134
نویسندگان
Naruyoshi Fujiwara, Jun Shimizu, Kenji Takai, Nagisa Arimitsu, Asako Saito, Takao Kono, Tasuku Umehara, Yuji Ueda, Sueshige Wakisaka, Tomoko Suzuki, Noboru Suzuki,