ADAM17 regulates self-renewal and differentiation of U87 glioblastoma stem cells

► CD133+ cells coexpress higher levels of ADAM17 in both U87 glioblastoma stem cells (GSCs) and primary human glioma samples. ► ADAM17 blockade depletes CD133-positive cells and inhibits neurosphere formation in GSCs. ► ADAM17 inhibition induces GSCs neurosphere differentiation. ► ADAM17 knockdown decreases Notch signaling pathway activation.