کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6288202 1615929 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular analysis of recombinase-mediated cassette exchange reactions catalyzed by integrase of coliphage HK022
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Molecular analysis of recombinase-mediated cassette exchange reactions catalyzed by integrase of coliphage HK022
چکیده انگلیسی
The integrase (Int) protein of coliphage HK022 can catalyze in Escherichia coli as well as in in vitro integrative and excisive recombinase-mediated cassette exchange reactions between plasmids as substrates. Atomic force microscopy images have revealed that in the protein-DNA complexes that are formed, the plasmid substrates are connected via one and not two pairs of attachment sites. This observation, together with the elucidation of intermediate co-integrates between the two circular plasmids, suggest that a sequential mechanism of the RMCE reaction is possible.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 159, Issues 9–10, November–December 2008, Pages 663-670
نویسندگان
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