کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6290138 1616618 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Addition of ethanol to supercritical carbon dioxide enhances the inactivation of bacterial spores in the biofilm of Bacillus cereus
ترجمه فارسی عنوان
اضافه کردن اتانول به دی اکسید کربن فوق بحرانی باعث افزایش غلظت اسپور باکتریایی در بیوفیلم باسیلوس سرئوس
کلمات کلیدی
دی اکسید کربن فوق بحرانی، استریلیزاسیون، اسپور، بیوفیلم، همکاران
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی
Supercritical carbon dioxide (SC-CO2) was used to inactivate Bacillus cereus spores inside biofilms, which were grown on stainless steel. SC-CO2 treatment was tested using various conditions, such as pressure treatment (10-30 MPa), temperature (35-60 °C), and time (10-120 min). B. cereus vegetative cells in the biofilm were completely inactivated by treatment with SC-CO2 at 10 MPa and at 35 °C for 5 min. However, SC-CO2 alone did not inactivate spores in biofilm even after the treatment time was extended to 120 min. When ethanol was used as a cosolvent with SC-CO2 in the SC-CO2 treatment using only 2-10 ml of ethanol in 100 ml of SC-CO2 vessel for 60-90 min of treatment time at 10 MPa and 60 °C, B. cereus spores in the biofilm were found to be completely inactivated in the colony-forming test. We also assessed the viability of SC-CO2-treated bacterial spores and vegetative cells in the biofilm by staining with SYTO 9 and propidium iodide. The membrane integrity of the vegetative cells was completely lost, while the integrity of the membrane was still maintained in most spores. However, when SC-CO2 along with ethanol was used, both vegetative cells and spores lost their membrane integrity, indicating that the use of ethanol as a cosolvent with SC-CO2 is efficient in inactivating the bacterial spores in the biofilm.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 166, Issue 2, 2 September 2013, Pages 207-212
نویسندگان
, , , ,