Pyrosequencing for rapid molecular identification of Schistosoma japonicum and S. mekongi eggs and cercariae

Alignment of the 18S nbosomal RNA gene derived from S. japonicum and S. mekongi. The dotted arrows indicate the position of Schis_F (forward primer) and biotinylated Schis_R (reverse primer) for amplification. A solid arrow indicates Schis_S (sequencing primer), and a rectangular box shows the position of the target region used for species level identification. Dots indicate identical nucleotides between lines.