Besnoitia besnoiti protein disulfide isomerase (BbPDI): Molecular characterization, expression and in silico modelling

► Besnoitia besnoiti expresses a 471 amino acid protein disulfide isomerase (PDI). ► B. besnoiti PDI (BbPDI) belongs to the thioredoxin like superfamily. ► Phylogenetically, PDI from B. besnoiti, Neospora caninum and Toxoplasma gondii form a cluster. ► A BbPDI 3D theoretical model was built by comparative homology. ► Recombinant BbPDI shows a dose-dependent activity and is inhibited by bacitracin.