کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6292219 | 1302520 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hymenolepis diminuta: Mitochondrial transhydrogenase as an additional site for anaerobic phosphorylation
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کلمات کلیدی
ATP generationDCCDNADH oxidaseFCCPHymenolepis diminutaCCCPBSA - BSACestode - CestodaN,N′-dicyclohexylcarbodiimide - N، N'-dicyclohexylcarbodiimidebovine serum albumin - آلبومین سرم گاوEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدElectron transport - انتقال الکترونTris–HCl - تریس-HClSMP - دانشکده دبیرستانSubmitochondrial particles - ذرات ریزMitochondrial inner membrane - غشای داخلی میتوکندریایfumarate reductase - فومارات ردوکتازMitochondria - میتوکندریاCarbonyl cyanide 3-chlorophenylhydrazone - کربنیل سیانید 3-کلروفنیل هیدرازونcarbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone - کربونیل سیانید 4- (trifluoromethoxy) phenylhydrazone
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Hymenolepis diminuta: Mitochondrial transhydrogenase as an additional site for anaerobic phosphorylation Hymenolepis diminuta: Mitochondrial transhydrogenase as an additional site for anaerobic phosphorylation](/preview/png/6292219.png)
چکیده انگلیسی
Employing adult Hymenolepis diminuta SMP and exogenous pyridine nucleotide-generating systems, reduced pyridine nucleotide-dependent net 32P incorporation into ATP was examined. NADH supported rotenone-sensitive 32P incorporation and this rate increased markedly with fumarate addition, in keeping with an active fumarate reductase. Interestingly, corresponding evaluations with NADPH did not result in detectable phosphorylation in the absence or presence of fumarate. However, with NAD addition, but without NAD generation, active NADPH-dependent phosphorylation occurred, thereby demonstrating mitochondrial transhydrogenase involvement, and 32P incorporation increased significantly with fumarate addition. More importantly, in the presence of rotenone and both NADPH and NAD generation, significant net 32P incorporation was noted, but was undetectable in the presence of DCCD or protonophores (e.g., niclosamide). Without NAD generation, minimal phosphorylation occurred. These data demonstrate that with ongoing NADPH and NAD generation, the H. diminuta, proton-translocating, mitochondrial transhydrogenase can serve as an additional anaerobic phosphorylation site. A model is presented.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 119, Issue 1, May 2008, Pages 24-29
Journal: Experimental Parasitology - Volume 119, Issue 1, May 2008, Pages 24-29
نویسندگان
Nancy Mercer-Haines, Carmen F. Fioravanti,