کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6308089 | 1618847 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Multixenobiotic resistance efflux activity in Daphnia magna and Lumbriculus variegatus
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کلمات کلیدی
Multidrug resistance related proteinqRT-PCRMRPLumbriculus variegatusrHbP-gpNBDMDRCalcein-AMMXRDaphnia magna - دافنی مگنا nucleotide binding domain - دامنه اتصال نوکلئوتیدیRhodamine B - رودامین بquantitative reverse transcription PCR - رونویسی معکوس PCR کمیMultixenobiotic resistance - مقاومت چند اکسیوبیویتیکABC protein - پروتئین ABCmultidrug resistance protein - پروتئین مقاوم در برابر چندین رژیمPermeability glycoprotein - گلیکوپروتئین نفوذپذیری
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Multixenobiotic resistance is a phenomenon in which ATP-binding cassette (ABC) family proteins transfer harmful compounds out of cells. Daphnia magna and Lumbriculus variegatus are model species in aquatic ecotoxicology, but the presence and activity of ABC proteins have not been well described in these species. The aim of this work was to study the presence, activity, and inhibition of ABC transport proteins in D. magna and L. variegatus. The presence of abcb1 and abcc transcripts in 8-9-day-old D. magna was investigated by qRT-PCR. The activity of MXR in D. magna and L. variegatus was explored by influx of the fluorescent ABC protein substrates rhodamine B and calcein-AM, with and without the model inhibitors verapamil (unspecific ABC inhibitor), reversin 205 (ABCB1 inhibitor) and MK571 (ABCC inhibitor). Juvenile D. magna possessed all examined abcb and abcc transcripts, but only reversin 205 inhibited MXR activity. The MXR activity in L. variegatus was inhibited by MK571, and to a lesser extent by verapamil, whereas reversin 205 seemed to stimulate the transport activity. Whereas calcein-AM worked better as an MXR substrate in D. magna, rhodamine B was a better substrate for L. variegatus MXR activity measurements. This is the first report on MXR activity in the order Lumbriculida, subclass Oligochaeta, and class Clitellata.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 124, April 2015, Pages 143-149
Journal: Chemosphere - Volume 124, April 2015, Pages 143-149
نویسندگان
Eeva-Riikka Vehniäinen, Jussi V.K. Kukkonen,