کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6308344 1618853 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interaction of 2-(2′,4′-bromophenoxyl)-benzoquinone with deoxynucleosides and DNA in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Interaction of 2-(2′,4′-bromophenoxyl)-benzoquinone with deoxynucleosides and DNA in vitro
چکیده انگلیسی
Polybrominated diphenyl ethers (PBDEs) may be metabolized to form hydroxylated and quinone products. Study on the formation of DNA adducts altered by PBDEs quinones was conducted. Various types of DNA adducts generated from in vitro reaction of deoxyguanosine (dG), 2′-deoxyadenosine (dA), 2′-deoxycytidine (dC), thymidine (T) and DNA with a PBDE-quinone metabolite, namely 2-(2′,4′-bromophenoxyl)-benzoquinone (2′4′BrPhO-BQ) were characterized. The results suggest that the quinone compound could form various DNA adducts with dG, dA and dC via Michael Addition, which was confirmed from analyses by electrospray ionization tandem mass spectrometry. Two adducts were respectively generated from the reactions of 2′4′BrPhO-BQ with dC and dG, while three adducts were produced with dA. The formation of adducts of 2′4′BrPhO-BQ-deoxynucleoside changed with different pH of reaction solution. The obtained results demonstrated that 2′4′BrPhO-BQ could covalently bind to DNA mediated by quinone group. The in vitro data of the formation of DNA adducts might be valuable to elucidate the mechanism of interaction between PBDEs and DNA in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 118, January 2015, Pages 29-34
نویسندگان
, , , , ,