Eucalyptus tolerance mechanisms to lanthanum and cerium: Subcellular distribution, antioxidant system and thiol pools

- La and Ce accumulation in eucalyptus were 696.5 and 493.7 mg plant−1, respectively.
- Cell walls stored 45.40-63.44% of the metals under La or Ce stress.
- POD and CAT activity enhanced at low La or Ce; but no change in GSH or AsA.
- NPT concentration increased at low La or Ce, PCs concentration continued to increase under La or Ce stress.

Guanglin 9 (Eucalyptus grandis × Eucalyptus urophlla) and Eucalyptus grandis 5 are two eucalyptus species which have been found to grow normally in soils contaminated with lanthanum and cerium, but the tolerance mechanisms are not clear yet. In this study, a pot experiment was conducted to investigate the tolerance mechanisms of the eucalyptus to lanthanum and cerium. Cell walls stored 45.40-63.44% of the metals under lanthanum or cerium stress. Peroxidase and catalase activities enhanced with increasing soil La or Ce concentrations up to 200 mg kg−1, while there were no obvious changes in glutathione and ascorbate concentrations. Non-protein thiols concentrations increased with increasing treatment levels up to 200 mg kg−1, and then decreased. Phytochelatins concentrations continued to increase under La or Ce stress. Therefore, the two eucalyptus species are La and Ce tolerant plants, and the tolerance mechanisms include cell wall deposition, antioxidant system response, and thiol compound synthesis.