کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6309401 | 1645519 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Toxicity of copper oxide nanoparticles in the blue mussel, Mytilus edulis: A redox proteomic investigation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Toxicity of copper oxide nanoparticles in the blue mussel, Mytilus edulis: A redox proteomic investigation Toxicity of copper oxide nanoparticles in the blue mussel, Mytilus edulis: A redox proteomic investigation](/preview/png/6309401.png)
چکیده انگلیسی
Relatively little is known about the fate and effects of nanomaterials even in relatively simple organisms such as Mytilus edulis. Here, copper oxide nanoparticles (CuO NP) are shown to induce dose-dependent toxic effects at the biochemical, physiological and tissue levels in the blue mussel. Stable CuO NP suspensions were sized by differential light scattering and nanoparticle tracking analysis to yield average particle diameters of approximately 100Â nm. These were administered to M. edulis, at doses of 400, 700 and 1000Â ppb. Ingested copper was predominantly located in the gill tissue with small amounts in digestive gland. Fifteen coomassie-stained spots were excised from two dimensional gel electrophoresis separations of gill tissue extacts and identified by peptide mass fingerprinting. These contained six unique proteins (alpha- and beta-tubulin, actin, tropomyosin, triosephosphate isomerase and Cu-Zn superoxide dismutase). Of these, two spots (actin and triosephosphate isomerase) showed decreased protein thiols while three (alpha-tubulin, tropomyosin and Cu-Zn superoxide dismutase) showed increased carbonylation which is indicative of protein oxidation of cytoskeleton and enzymes in response to CuO NP. The neutral red retention time (NRRT) assay revealed toxicity due to the CuO NPs which was comparable with toxic metal oxide nanoparticles such as chromium and cobalt. In contrast, non-toxic titanium and gold metal oxide nanoparticles gave no NRRT effects at similar NP concentrations. Histology revealed deposition of pigmented brown cells in response to CuO NP, located predominantly along the mantle and gill margin but also lining digestive tubules and some of the sinuses and distributed throughout the connective tissue and in the adductor muscle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 108, August 2014, Pages 289-299
Journal: Chemosphere - Volume 108, August 2014, Pages 289-299
نویسندگان
Wentao Hu, Sarah Culloty, Grainne Darmody, Sharon Lynch, John Davenport, Sonia Ramirez-Garcia, Kenneth A. Dawson, Iseult Lynch, Julian Blasco, David Sheehan,