کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6309606 1307453 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Toxic effects of imidazolium-based ionic liquids on Caenorhabditis elegans: The role of reactive oxygen species
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Toxic effects of imidazolium-based ionic liquids on Caenorhabditis elegans: The role of reactive oxygen species
چکیده انگلیسی


- 24-h LC50 concentrations of 8 ionic liquids to C. elegans were determined.
- Significant increases of biomarkers (ROS and lipofuscin) were observed.
- DMSO remarkably rescued the lethality of worms and decreased the ROS level.
- ROS play an important role in IL-induced toxicity.

By using Caenorhabditis elegans (C. elegans) as a model animal, the present work is aimed to evaluate the acute toxicity of imidazolium-based bromide Ionic Liquids (ILs), and to elucidate the underlying mechanisms involved. Firstly, 24-h median lethal concentration (LC50) for eight ILs with different alkyl chain lengths and one or two methyl groups in the imidazolium ring were determined to be in a range of 0.09-6.64 mg mL−1. Four ILs were selected to investigate the toxic mechanisms. Mortality, levels of reactive oxygen species (ROS), lipofuscin accumulation and expression of superoxide dismutase 3 in C. elegans were determined after exposed to ILs at sub-lethal concentrations for 12 h. A significant increase in the levels of these biomarkers was observed in accordance with the results of 12-h lethality assay. The addition of 0.5% dimethyl sulfoxide, which acts as a radical scavenger, remarkably rescued the lethality of C. elegans and significantly decreased the ROS level in C. elegans. Our results suggest that ROS play an important role in IL-induced toxicity in C. elegans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 93, Issue 10, November 2013, Pages 2399-2404
نویسندگان
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