کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6310368 1307465 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New insights for the risk of bisphenol A: Inhibition of UDP-glucuronosyltransferases (UGTs)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
New insights for the risk of bisphenol A: Inhibition of UDP-glucuronosyltransferases (UGTs)
چکیده انگلیسی


- Stronger inhibition of bisphenol A towards UGT2B isoforms than UGT1A isoforms.
- Competitive inhibition for UGT2B4, noncompetitive inhibition for 2B7, 2B15, 2B17.
- In vitro-in vivo extrapolation (IV-IVE) was performed.

Bisphenol A (BPA), the important endocrine-disrupting chemical (EDC), has been reported to be able to induce various toxicity. The present study aims to understand the toxicity behavior of bisphenol A through evaluating the inhibition profile of bisphenol A towards UDP-glucuronosyltransferase (UGT) isoforms. In vitro recombinant UGTs-catalyzed 4-methylumbelliferone (4-MU) glucuronidation reaction was employed as probe reaction for all the tested UGT isoforms. The results showed that bisphenol A exerted stronger inhibition towards UGT2B isoforms than UGT1A isoforms. Furthermore, the inhibition kinetic type and parameters (Ki) were determined for the inhibition of bisphenol A towards UGT2B4, 2B7, 2B15, and 2B17. Bisphenol A exhibited the competitive inhibition towards UGT2B4, and noncompetitive inhibition towards UGT2B7, 2B15 and 2B17. The inhibition kinetic parameters (Ki) were calculated to be 1.1, 32.6, 5.6, and 19.9 μM for UGT2B4, 2B7, 2B15 and 2B17, respectively. In combination with the in vivo concentration of bisphenol A, the elevation of exposure dose was predicted to increase by 29.1%, 1%, 5.7%, and 1.6% for UGT2B4, 2B7, 2B15, and 2B17, indicating the high influence of bisphenol A towards the in vivo UGT2B isofroms-mediated metabolism of xenobiotics and endogenous substances. All these data provide the supporting information for deeper understanding of toxicology of bisphenol A.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 93, Issue 6, October 2013, Pages 1189-1193
نویسندگان
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