کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6311398 1307510 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effluent from drug manufacturing affects cytochrome P450 1 regulation and function in fish
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Effluent from drug manufacturing affects cytochrome P450 1 regulation and function in fish
چکیده انگلیسی

We have previously reported very high concentrations of pharmaceuticals in the effluent from a treatment plant receiving wastewater from about 90 bulk drug manufacturers near Hyderabad, India. The main objective of the present study was to examine how high dilutions of this effluent affect mRNA expression of cytochrome P450 (CYP) 1 family genes and ethoxyresorufin O-deethylase (EROD) activity in exposed wildlife, using the three-spined stickleback (Gasterosteus aculeatus) as a model. In gill filaments exposed to diluted effluent ex vivo, EROD activity was strongly inhibited in a concentration-dependent manner. In a subsequent in vivo study, groups of fish were exposed (24 h) to three concentrations of effluent, 0.8%, 1.6% or 3.2%. In this experiment, EROD in gills was induced 27-, 52- or 60-fold, respectively. Accordingly, CYP1A mRNA was markedly up-regulated in gill, liver and brain of fish exposed to all three effluent concentrations. Expression of mRNA for CYP1B1 and CYP1C1 was induced in gills at all concentrations while effects on these genes in liver and brain were weak or absent. The results of a time course study suggested that most CYP1-inducing substances in the effluent were readily metabolised or excreted, because the induced EROD activity and mRNA expression decreased when the fish were transferred to clean water. Considering that CYP1 enzymes play important roles in biotransformation of endogenous and foreign compounds, the observed dual effect of the effluent on CYP1 catalytic activity and mRNA expression suggests that multiple physiological functions could be affected in exposed wildlife.

► Treated wastewater from bulk drug manufacturing was examined using molecular biomarkers in fish. ► Diluted effluent inhibited gill EROD activity ex vivo. ► Diluted effluent induced gill EROD activity and expression of CYP1 mRNAs in gill, liver and brain. ► Results suggest a risk for down-stream effects on CYP1 regulated physiological functions in exposed wildlife.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 90, Issue 3, January 2013, Pages 1149-1157
نویسندگان
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